所属单位：化学与生命科学学院

教研室：生物技术教研室

发表刊物：Proceedings of the National Academy of Sciences of the United States of America

项目来源：国家自然科学基金项目

关键字：circular dichroism; DNA conformation; i-motif DNA; ligand–DNA interactions

摘要：As the leading nanodevice candidate, single-walled carbon nano-tubes (SWNTs) have potential therapeutic applications in gene therapy and novel drug delivery. We found that SWNTs can inhibit DNA duplex association and selectively induce human telomeric i-motif DNA formation by binding to the 5'-end major groove under physiological conditions or even at pH 8.0. SWNT binding to telomeric DNA was studied by UV melting, NMR, S1 nuclease cleavage, CD, and competitive FRET methods. These results suggest that SWNTs might have the intriguing potential to modulate human telomeric DNA structures in vivo, like biologically relevant B-A and B-Z DNA transitions, which is of great interest for drug design and cancer therapy.

第一作者：李奚

论文类型：期刊论文

卷号：103

期号：52

页面范围：1

字数：1

ISSN号：0027-8424

是否译文：否

发表时间：2006-10-26