所属单位：化学与生命科学学院

教研室：生物技术教研室

发表刊物：Nucleic Acids Research

项目来源：国家自然科学基金项目

关键字：FLOW LINEAR DICHROISM; CIRCULAR-DICHROISM; BINDING; SEQUENCE; CONDENSATION; POLYMORPHISM; RECOGNITION; NEOMYCIN; AGENTS; FORM

摘要：Single-walled carbon nanotubes (SWNTs) have been considered as the leading candidate for nano-device applications ranging from gene therapy and novel drug delivery to membrane separations. The miniaturization of DNA-nanotube devices for biological applications requires fully understanding DNA-nanotube interaction mechanism. We report here, for the first time, that DNA destabilization and conformational transition induced by SWNTs are sequence-dependent. Contrasting changes for SWNTs binding to poly[dGdC]:poly[dGdC] and poly[dAdT]:poly[dAdT] were observed. For GC homopolymer, DNA melting temperature was decreased 40 degrees C by SWNTs but no change for AT-DNA. SWNTs can induce B-A transition for GC-DNA but AT-DNA resisted the transition. Our circular dichroism, competitive binding assay and triplex destabilization studies provide direct evidence that SWNTs induce DNA B-A transition in solution and they bind to the DNA major groove with GC preference.

第一作者：李奚

论文类型：期刊论文

卷号：34

期号：13

页面范围：1

字数：1

ISSN号：0305-1048

是否译文：否

发表时间：2006-07-05