Supervisor of Doctorate Candidates
Supervisor of Master's Candidates
Hits:
Affiliation of Author(s):化学与生命科学学院
Teaching and Research Group:生物技术教研室
Journal:Nucleic Acids Research
Funded by:国家自然科学基金项目
Key Words:FLOW LINEAR DICHROISM; CIRCULAR-DICHROISM; BINDING; SEQUENCE; CONDENSATION; POLYMORPHISM; RECOGNITION; NEOMYCIN; AGENTS; FORM
Abstract:Single-walled carbon nanotubes (SWNTs) have been considered as the leading candidate for nano-device applications ranging from gene therapy and novel drug delivery to membrane separations. The miniaturization of DNA-nanotube devices for biological applications requires fully understanding DNA-nanotube interaction mechanism. We report here, for the first time, that DNA destabilization and conformational transition induced by SWNTs are sequence-dependent. Contrasting changes for SWNTs binding to poly[dGdC]:poly[dGdC] and poly[dAdT]:poly[dAdT] were observed. For GC homopolymer, DNA melting temperature was decreased 40 degrees C by SWNTs but no change for AT-DNA. SWNTs can induce B-A transition for GC-DNA but AT-DNA resisted the transition. Our circular dichroism, competitive binding assay and triplex destabilization studies provide direct evidence that SWNTs induce DNA B-A transition in solution and they bind to the DNA major groove with GC preference.
First Author:li xi
Indexed by:Journal paper
Volume:34
Issue:13
Page Number:1
Number of Words:1
ISSN No.:0305-1048
Translation or Not:no
Date of Publication:2006-07-05
