Supervisor of Doctorate Candidates
Supervisor of Master's Candidates
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Affiliation of Author(s):化学与生命科学学院
Teaching and Research Group:生物技术教研室
Journal:Proceedings of the National Academy of Sciences of the United States of America
Funded by:国家自然科学基金项目
Key Words:circular dichroism; DNA conformation; i-motif DNA; ligand–DNA interactions
Abstract:As the leading nanodevice candidate, single-walled carbon nano-tubes (SWNTs) have potential therapeutic applications in gene therapy and novel drug delivery. We found that SWNTs can inhibit DNA duplex association and selectively induce human telomeric i-motif DNA formation by binding to the 5'-end major groove under physiological conditions or even at pH 8.0. SWNT binding to telomeric DNA was studied by UV melting, NMR, S1 nuclease cleavage, CD, and competitive FRET methods. These results suggest that SWNTs might have the intriguing potential to modulate human telomeric DNA structures in vivo, like biologically relevant B-A and B-Z DNA transitions, which is of great interest for drug design and cancer therapy.
First Author:li xi
Indexed by:Journal paper
Volume:103
Issue:52
Page Number:1
Number of Words:1
ISSN No.:0027-8424
Translation or Not:no
Date of Publication:2006-10-26
