点击次数：

所属单位：化学与生命科学学院

教研室：食品教研室

发表刊物：Journal of Separation Science

项目来源：其他课题

关键字：Cordyceps sinensis / HPLC / Matrix solid-phase dispersion / Nucleobases

摘要：Nine nucleosides and nucleobases, including uracil, adenine, thymine, uridine, adenosine,thymidine, cytidine, guanosine, and cordycepin in natural Cordyceps sinensis, cultured Cordyceps mycelia, and Cordyceps fruiting bodies were extracted by matrix solid-phase dispersion (MSPD) and determined by HPLC. The experimental conditions for the MSPD extraction were optimized. Florisil was used as dispersant, petroleum ether as washing solvent, and methanol as elution solvent. The Florisil-to-sample ratio was selected to be 4:1 and no additional clean-up sorbent was needed. The calibration curves had good linear relationships (r > 0.9997). The LOD and LOQ were in the range of 12 ∼ 79 and 41 ∼ 265 ng/mL,respectively. The intra- and interday precision were lower than 8.3%. The recoveries were between 61.5 and 93.2%. The present method consumed less sample compared with ultrasonic extraction and heating reflux extraction (HRE). The extraction yields obtained by using the present method are much higher than those obtained by UE and comparable to those obtained by HRE.

合写作者：李娜,王敏,王月,杜琳,季晓峰,于爱民,张寒琦,邱芳萍

第一作者：王志兵

论文类型：期刊论文

卷号：36

期号：14

页面范围：2

字数：1

是否译文：否

发表时间：2013-07-01