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Affiliation of Author(s):化学与生命科学学院
Teaching and Research Group:食品教研室
Journal:Journal of Chromatography B
Funded by:其他课题
Key Words:Silica-supported ionic liquid-based matrixsolid phase dispersion (S-SIL-based MSPD)；Phenolic acids and flavonoids；Raw propolis；High-performance liquid chromatography(HPLC)
Abstract:tThe silica-supported ionic liquid (S-SIL) was prepared by impregnation and used as the dispersion adsor-bent of matrix solid phase dispersion (MSPD) for the simultaneous extraction of eight phenolic acidsand flavonoids, including caffeic acid, ferulic acid, morin, luteolin, quercetin, apigenin, chrysin, andkaempferide in raw propolis. High performance liquid chromatography with a Zorbax SB-C18 column(150 mm × 4.6 mm, 3.5 m) was used for separation of the analytes. The mobile phase consisted of 0.2%phosphoric acid aqueous solution and acetonitrile and the flow rate of the mobile phase was 0.5 mL/min.The experimental conditions for silica-supported ionic liquid-based matrix solid phase dispersion (S-SIL-based MSPD) were optimized. S-SIL containing 10% [C6MIM]Cl was used as dispersant, 20 mL of n-hexaneas washing solvent and 15 mL of methanol as elution solvent. The ratio of S-SIL to sample was selectedto be 4:1. The standard curves showed good linear relationship (r > 0.9995). The limits of detection andquantification were in the range of 5.8–22.2 ng mL−1and 19.2–74.0 ng mL−1, respectively. The relativestandard deviations (RSDs) of intra-day and inter-day determination were lower than 8.80% and 11.19%,respectively. The recoveries were between 65.51% and 92.32% with RSDs lower than 8.95%. Comparedwith ultrasound-assisted extraction (UAE) and soxhlet extraction, the present method consumed lesssample, organic solvent, and extraction time, although the extraction yields obtained by S-SIL-basedMSPD are slightly lower than those obtained by UAE
Co-author:孙睿,王远鹏,李娜,雷蕾,杨潇,于爱民,张寒琦,邱芳萍
First Author:Zhibing Wang
Indexed by:Journal paper
Volume:969
Page Number:2
Number of Words:1
Translation or Not:no
Date of Publication:2014-10-15