【SCI通讯】Self-assembled raccoon dog parvovirus VP2 protein confers immunity against RDPV disease in raccoon dogs:in vitro and in vivo studies

Release time:2021-07-05  Hits:

• Affiliation of Author(s):化学与生命科学学院
• Teaching and Research Group:化学与生命科学学院
• Journal:Viroiogy Journal
• Funded by:省、市、自治区科技项目
• Key Words:Raccoon dog parvovirus, Virus like particle, Trigger factor 16, Escherichia coli, Immunogenicity
• Abstract:Abstract Background: Raccoon dog parvovirus (RDPV) causes acute infectious diseases in raccoon dogs and may cause death in severe cases. The current treatment strategy relies on the extensive usage of classical inactivated vaccine which is marred by large doses, short immunization cycles and safety concerns. Methods: The present study aimed at optimization of RDPV VP2 gene, subcloning the gene into plasmid pET30a, and its subsequent transfer to Escherichia coli with trigger factor 16 for co-expression. The protein thus expressed was purifed with ammonium sulfate precipitation, hydrophobic chromatography, and endotoxin extraction procedures. VLPs were examined by transmission electron microscopy, dynamic light scattering, and the efcacy of VLPs vaccine was tested in vivo. Results: Results indicated that RDPV VP2 protein could be expressed soluble. Transmission electron microscopy and dynamic light scattering results indicated that RDPV VP2 self-assembled into VLPs. Hemagglutination inhibition antibody titers elicited by Al(OH)3 adjuvanted RDPV VLPs were comparable with RDPV inactivated vaccines, and the viral loads in the blood of the struck raccoon dogs were greatly reduced. Hematoxylin and eosin and Immunohistochemical results indicated that RDPV VLPs vaccine could protect raccoon dogs against RDPV infections. Conclusions: These results suggest that RDPV VLPs can become a potential vaccine candidate for RDPV therapy
• Co-author:夏霖亚
• First Author:carter
• Indexed by:Journal paper
• Page Number:1
• Translation or Not:no
• Date of Publication:2021-04-15