【重要.学生第一】异柠檬酸裂解酶肽类抑制剂结构的修饰

Release time:2021-04-12  Hits:

• Affiliation of Author(s):化学与生命科学学院
• Teaching and Research Group:化学与生命科学学院
• Journal:吉林大学学报.理学版
• Funded by:省、市、自治区科技项目
• Key Words:异柠檬酸裂解酶, 肽类, 抑制剂, 环肽
• Abstract:利用Fmoc固相多肽合成法, 按已知异柠檬酸裂解酶抑制剂的直链肽氨基酸序列合成首尾相连的环肽抑制剂. 经色谱纯化和质谱鉴定, 其相对分子质量的实测值与理论值相符. 抑制率实验结果表明, 合成的环肽对异柠檬酸裂解酶有明显抑制作用, 抑制率大于50%. 采用高效液相色谱法分别检测直链肽和环肽在血浆中的半衰期, 实验 结果表明, 环肽在血浆中的半衰期为11 min, 比直链肽的半衰期延长175%. 服务 把本文推荐给朋友 加入我的书架 加入引用管理器 E-mail Alert RSS 作者相关文章 刘志远 张爱臣 刘新涛 关晓侠 李玲玲 殷玉和 关键词 ： 异柠檬酸裂解酶, 肽类, 抑制剂, 环肽 Abstract： Fmoc solidphase peptide synthesis was used to synthesize cyclic peptide in light of the known isocitrate lyase linear peptide sequence according to the end to end way. Mass spectrometry demonstrates that the measured value of relative molecular mass is consistent with the theoretical value. Inhibition test confirms that the cyclic peptide shows significant inhibition to the ICL activity (the inhibition rate was more than 50%). At the same time, halflife of linear peptide and that of cyclic peptide in plasma were measured by HPLC. Results indicate that halflife of cyclic peptide in plasma amounts to 11 min, which is increased by 175% compared with that of linear peptide. Key words： isocitrate lyase peptide inhibitor cyclic peptide
• Co-author:刘志远
• First Author:carter
• Indexed by:Journal paper
• Volume:52
• Issue:4
• Page Number:3
• Number of Words:1
• Translation or Not:no
• Date of Publication:2014-04-01