郭志华

硕士生导师

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所在单位:制药工程系

职务:制药工程专业实验教师

学历:博士研究生毕业

办公地点:南湖校区化工楼

在职信息:在职

论文成果

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Enhanced Antisense Oligonucleotide Delivery Using Cationic Liposomes Incorporating FattyAcid-Modified Polyethylenimine

发布时间:2022-10-17 点击次数:

所属单位:化学工程学院
发表刊物:CURRENT PHARMAC- EUTICAL BIOTECHNOLOGY
项目来源:自选课题
摘要:Antisense oligonucleotides (ASOs) have promising therapeutic potential in oncotherapy. However, low stability and efficacy limit their application in the clinic. Cationic liposomes have been investigated as delivery vehicles for ASOs. Here, we report the synthesis and evaluation of an ASO delivery vehicle comprising cationic liposomes incorporating fatty acid-modified polyethylenimine. An oleic acid derivative of branched polyethylenimine (PEI-OA) and a linoleic acid derivative of branched polyethylenimine (PEI-LA) were synthesized and incorporated into liposomes. The PEI-modified liposomes were synthesized by an ethanol injection method with composition of PEI-modified lipid/Chol/TPGS. The properties of these liposomes, including cytotoxicity, cellular uptake, ASO target silencing activity, based on mRNA and protein downregulation, were investigated. LOR-2501, an ASOs targeting ribonucleotide reductase R1 subunit (R1) was used as the therapeutic cargo. The PEI-modified liposomes showed relatively compact particle size and excellent colloidal stability for at least 25 days. PEI-modified liposomes effectively delivered LOR-2501 into KB cells and efficiently induced down-regulation of R1 mRNA and protein. Compared with regular cationic liposomes, PEI-modified liposomes was more effective, reducing R1 mRNA and protein by ~10%.
合写作者:Yujing Li,Yige Fu,Tianqi Guo,Xin Li,Shuang Yang,Jing Xie
第一作者:郭志华
论文类型:期刊论文
卷号:15
期号:9
页面范围:2
是否译文:
发表时间:2014-02-28